Table 2 sgRNA amplification primers
From: Essential genes shape cancer genomes through linear limitation of homozygous deletions
sgRNA primer | Sequence |
|---|---|
Forward | 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT - (1–9 bp variable length sequence) - tcttgtggaaaggacgaaacaccg -3′ |
Reverse | 5′-CAAGCAGAAGACGGCATACGAGAT – 10 nt Index – GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTtgtgggcgatgtgcgctctg -3′ |
