Fig. 2
Abnormal axonal projections and glomerular domain organization in Bcl11b−/− mice. a–d Axonal projections of class I-OSNs and class II-OSNs were visualized using Olfr545-IRES-tauGFP mice (a, c) and Olfr151-IRES-taulacZ mice (b, d), respectively with control and Bcl11b−/− backgrounds. a Olfr545-expressing class I-OSNs project to ectopic and multiple glomeruli in the Bcl11b−/− OB (arrows). b Olfr151-expressing class II OSNs send their axon to a few glomeruli in the OB of control mice (arrowheads), while those of Bcl11b−/− mice are barely detected. c Axonal termini of Olfr545-expressing class I-OSNs were immunostained using anti-GFP (green) and anti-protocadherin (Pcdh) 21 (red) antibodies and counterstained with DAPI (blue). Numbers represent the section numbers of Olfr545-positive axonal termini observed in the consecutive coronal sections of OB. Arrowheads indicate Olfr545-positive glomeruli. d Axonal termini of Olfr151-expressing class II OSNs were immunostained using anti-β-gal (green) and anti-Pcdh21 (red) antibodies and counterstained with DAPI (blue). The section number of Olfr151-positive axonal termini observed were shown as in (d). Arrowheads indicate Olfr151-positive glomeruli. Arrow indicates Olfr151-positive axonal terminus that did not innervate into the glomerular layer. e Axonal projection domains of class I OSNs were visualized using ΔOlfr545-YFP mice with control and Bcl11b−/− backgrounds. Dotted lines indicate the OB outline. Coronal sections of the OB were immunostained as in (c). Dotted boxes are magnified. Arrowheads indicate ΔOlfr545-positive axonal termini in the ventral OB. f Axonal projection domains of class II OSNs in P-lacZ Tg mice with control and Bcl11b−/− backgrounds. Coronal sections of the OB were immunostained as in (d). Dotted boxes are magnified. D, dorsal; V, ventral; R, rostral; C, caudal; A, anterior; P, posterior. Scale bars, 100 μm
