Fig. 4

Bcl11b negatively regulates the expression of class I OR genes. a Genetic strategy of the gain-of-Bcl11b-function analysis in OSNs. Robust expression of Bcl11b throughout the MOE of the gain-of-function mutant mice was confirmed by IHC (Supplementary Fig. 5a). b ISH with mixed RNA probes for the four class I and dorsal class II genes in coronal sections of the MOE of the control and gain-of-function mutant mice. Scale bar, 100 μm. c Quantification of the number of OSNs expressing either class I or class II genes per section. Bars represent the mean values ± s.e.m. of the control (gray) and gain-of-function mutant mice (class I in blue, class II in magenta), respectively: Class I genes: 33.8 ± 1.60 in control and 3.78 ± 1.82 in mutant mice, p = 0.000243, two-tailed t-test, n = 3 independent experiments; Class II genes: 25.6 ± 1.57 in control and 26.4 ± 3.15 in mutant mice, p = 0.838, two-tailed t-test, n = 3 independent experiments. ****p < 0.001, NS, not significant. Quantification data and statistical details are summarized in Supplementary Data 1. d The log₂-fold change values of OR gene expression analyzed by RNA-seq are arranged according to their relative positions along the chromosomes. Class I genes (blue), atypical class I genes (purple), and class II genes (magenta). Increased- and decreased OR genes (p < 0.05) are represented by filled circles. FPKMs in the control and gain-of-function mutant MOEs are summarized in Supplementary Data 3. e Merged representation of the bee-swarm and box-plots of RNA-seq FPKM values for class I (n = 128) and class II genes (n = 968) of the control (gray) and gain-of-function mutant (class I genes in blue; class II genes in magenta) mice