Fig. 5
Bcl11b suppresses the enhancer activity of the J element. a Class I and class II enhancer activities were visualized using J-Venus and P-LacZ Tg mice in the presence or absence of Bcl11b by IHC against Venus and β-gal in coronal sections of the MOE of J-Venus and P-LacZ Tg mice, respectively. b Genetic strategy to analyze the functional relationship between Bcl11b and the J element. If there is no functional relationship between the induced Bcl11b and the J element, the OSN should be colored yellow by Venus (green) and mRFP (magenta). Alternatively, if the induced Bcl11b negatively regulates the J element, the expression of gapVenus will be turned off and OSNs will be colored magenta by mRFP. IHC analyses of class I OSN-specific expression of Venus in J-Cre-IRES-Venus Tg mice were shown in Supplementary Fig. 6. c, IHC against Venus (green) and RFP (magenta) in the MOE of J-Cre-IRES-Venus Tg mice without and with the CAGp-LSL-Bcl11b-IRES-mRFP transgene. Green and magenta arrows indicate Venus- and mRFP-single positive cells, respectively. Yellow arrowheads indicate Venus/mRFP-double positive cells. d Merged representation of the bee-swarm and box-plots for the relative position of OSNs along the basal-apical axis (0–1.0) of the MOE. Venus-positive cells (433 cells in control, 200 cells in double Tg mutant) and/or RFP-positive cells (251 cells in double Tg mutant) collected from three animals of each mutant strain. Quantification data are summarized in Supplementary Data 1. Scale bars, 100 μm in (a); 50 μm in (c)
