Fig. 2
Lys242 SUMOylation and the SWSSS motif contribute to interaction between Tpz1 and Stn1-Ten1. a Southern blot analysis of telomere length for indicated mutant strains. All samples were prepared from strains that were extensively restreaked (>150 generations) on YES plates to ensure that terminal telomere lengths were achieved. b Y2H assay to monitor interaction between Tpz1 and Stn1-Ten1. The Gal4-activation domain (GAD) was fused to Tpz1, and the Gal4 DNA-binding domain (GBD) was fused to Stn1 and monitored for interaction in the presence of untagged Ten1. Growth on a –His plate indicates interaction. For GBD-Stn1 + Ten1, spots containing 1:5 dilutions are also shown. c Examination of Tpz1-Stn1 interaction by co-IP. Cdc2 western blot served as loading control for whole cell extract (WCE). Western blot signals were quantified to determine Stn1/Tpz1 ratio (normalized to wild-type) for FLAG-IP and WCE samples, and shown below blots. Molecular weight (kDa) of size markers are indicated
