Fig. 4

Effects of K242R and AWAAA mutations on cell-cycle-regulated recruitment of Trt1 at telomeres. a A model depicting replicating telomeres in fission yeast^62,63. b Summary of previous cell-cycle-ChIP results for DNA Polymerases, Trt1, Tpz1, Ccq1, Poz1, and Stn1²⁸. A proposed model for telomere length regulation pathway^22,28 is also indicated. c Cell-cycle-ChIP assays to monitor telomere binding of Trt1^TERT. Synchronized cell cultures were obtained using cdc25-22, and binding of myc-tagged Trt1 was monitored by dot-blot ChIP assays. Previously determined peaks of binding for leading (Polε) and lagging (Polα) strand DNA polymerases are indicated with gray shaded area^28,63. DNA Polε binding peaks at 120 min after release from G₂/M arrest for both wild-type and poz1∆ cells, while DNA Polα binding peaks at 140 min for wt cells and at 180 min for poz1∆ cells^28,63. A plot for % septated cells to monitor cell-cycle progression is also shown. Plots show mean values plus/minus SEM from at least three independent experiments⁶⁴. Raw data values for ChIP assays and % septated cells are shown in Supplementary Data 1