Fig. 6
Characterization of strains expressing Tpz1-Stn1 fusion protein. a Schematic representation of the Tpz1-Stn1 fusion construct. b, d Southern blot analysis of telomeres for indicated strains. All samples, except for stn1∆ and stn1∆ tpz1-stn1-E132R, were prepared from strains that were extensively restreaked (>150 generations) on YES plates. For stn1∆ and stn1∆ tpz1-stn1-E132R strains in b, hybridization signals for telomeric repeats are completely lost due to chromosome circularization. c Pulsed-field gel analysis of telomeric NotI-fragments. e, f Quantitative dot-blot ChIP assays to monitor telomere localization for e Stn1 and f Ten1. Protein expression levels for indicated myc-tagged proteins were monitored by western blot, with Cdc2 as a loading control. Molecular weight (kDa) of size markers are indicated. Plots show mean values plus/minus SEM and distribution of individual data points from at least six independent experiments. Raw data values and statistical analysis of ChIP assays by two-tailed Student’s t-test are shown in Supplementary Data 1
