Fig. 7
Expression of Tpz1-Stn1 fusion protein can protect telomeres in tpz1-K242R-AWAAA cells but fails to restore wild-type telomere length in tpz1-K242R-AWAAA or tpz1-AWAAA cells. a Southern blot analysis of telomere length for indicated mutant strains to test the ability of Tpz1-Stn1 fusion proteins to rescue telomere loss. b Pulsed-field gel analysis of telomeric fusions for strains carrying Tpz1-Stn1 fusion proteins. c Southern blot analysis to examine requirement of telomerase (Trt1) or HR repair proteins (Rad51 and Rad52) for telomere maintenance in tpz1-K242R-AWAAA-stn1 cells. d Southern blot analysis to examine effect of catalytically dead telomerase (trt1-D743A) in tpz1-K242R-AWAAA-stn1 cells. e Southern blot analysis of indicated tpz1 mutant strains with or without Tpz1-Stn1 fusion protein. f Southern blot analysis to test effect of trt1∆ or trt1-D743A for indicated strains. For Southern blots in a, c and e, all samples, except for tpz1-AWAAA-stn1-E132R, were prepared from strains that were extensively restreaked (>150 generations) on YES plates. For Southern blots in d and f, DNA was prepared from early (~30) or late (>150) generations after desired strains were generated by meiotic crosses for trt1 mutant cells. Right angle triangles were used to signifiy pair of early (left) and late (right) generation for these trt1 mutant strains
