Fig. 8
Effects of Tpz1-Stn1 K242R and AWAAA mutations on recruitment of various telomere-related factors to telomeres. a, b, d–f Dot-blot ChIP assays from asynchronous cell cultures to monitor binding of a Ten1, b Po11 (DNA Polα), d Rad11 (RPA), e Rad26ATRIP, and f Trt1TERT at telomeres. Expression levels of myc- or FLAG-tagged proteins used in ChIP assays were monitored by western blot analysis. Anti-Cdc2 blots served as loading control. Molecular weight (kDa) of size markers are indicated. Plots show mean values plus/minus SEM and distribution of individual data points from at least three independent experiments. Raw data values and statistical analysis of ChIP assays by two-tailed Student’s t-test are shown in Supplementary Data 1. c Interaction of Stn1 and Po11 (DNA Polα), detected by co-IP analysis
