Fig. 2
From: TRAF6 maintains mammary stem cells and promotes pregnancy-induced mammary epithelial cell expansion
TRAF6 is essential for pregnancy-induced alveologenesis. a Whole-mount analysis of the mammary gland in #2 fat pads of recipient wild-type mice and the outgrowths derived from transplanted TRAF6-He and TRAF6-KO epithelia in cleared fat pads at 8 weeks after transplantation. Scale bars, 500 μm (upper panels) or 1 cm (lower panels). b Hematoxylin and eosin (H&E) staining of the outgrowths in cleared fat pads of recipient mice. Scale bars, 200 μm. c Immunostaining of outgrowths in cleared fat pads of recipient mice with antibodies specific to Keratin 5 (Krt5, red) and E-cadherin (E-cad, green). Nuclei were stained with Hoechst 33342 (blue). Scale bars, 100 μm. d Luminal and basal cell populations in outgrowths at virgin (8 weeks after transplantation), P14 and L1 were analyzed by flow cytometry. Values represent the average ± SD (virgin, n = 3; P14, n = 4; L1, n = 3). e Whole-mount analysis of the mammary gland in #2 fat pads of recipient wild-type mice and the outgrowths of transplanted TRAF6-He and TRAF6-KO epithelia in cleared fat pads at P14 and L1. Scale bars, 500 μm
