Fig. 4
From: TRAF6 maintains mammary stem cells and promotes pregnancy-induced mammary epithelial cell expansion
TRAF6 is not essential for pregnancy-induced luminal cell maturation and milk production. a Real-time RT-qPCR analysis of Traf6 expression in luminal and basal cells isolated from outgrowths developed from TRAF6-He and TRAF6-KO epithelia in recipient mice in virgin stage, P14 and L1. Values are means ± SD (Virgin, n = 3; P14, n = 4; L1, n = 3; *p < 0.05). b Real-time RT-qPCR analysis of Csn2 and Wap expression in luminal and basal cells isolated from outgrowths developed from TRAF6-He and TRAF6-KO epithelia in recipient in virgin L1. Values are means ± SD (Virgin and L1, n = 3/group). c Immunostaining of outgrowths in cleared fat pads of recipient mice in virgin and L1 stages using milk-specific antibody. Nuclei were stained with hematoxylin. Scale bars, 200 μm
