Fig. 6
From: TRAF6 maintains mammary stem cells and promotes pregnancy-induced mammary epithelial cell expansion
TRAF6 selectively activates canonical NF-κB and AKT pathways in mammary epithelial cells during pregnancy. a Western blotting analysis of IκBα, p100, p52, phosphorylated AKT (p-AKT), and AKT expression in luminal and basal cells isolated from the mammary gland in #2 fat pads of recipient WT mice (#1) and the outgrowths derived from transplanted TRAF6-He and TRAF6-KO epithelia in cleared fat pads at P14. Tr: Transplanted fat pad; Re: Recipient’s fat pad. b Real-time RT-qPCR analysis of Nfkbia (IκBα mRNA) expression in luminal and basal cells isolated from outgrowths in recipient mice. Values are means ± SD (Virgin, n = 3; P14, n = 4; L1, n = 3; *p < 0.05 and **p < 0.01)
