Fig. 5
SNAT1 activates mTORC1 in cerebral neurons. a Procedure for deleting Slc38a1 using an in vitro culture system. Primary neurons isolated from the cerebral cortices of Slc38a1flox/flox mice were infected with a lentivirus encoding inactive ΔCre or active Cre, and control or Slc38a1-null neurons were prepared. b, c Deletion efficiency of Slc38a1 from primary neurons. Total RNA and protein were extracted from control or Slc38a1-null neurons, and the expression levels of Slc38a1 were assessed using qRT-PCR (b, n = 3) or western blotting (c, n = 3). d–f Analysis of mTORC1 activity after Slc38a1 deficiency. Proteins were extracted from control or Slc38a1-null neurons, and pp70S6k1(T389), pmTOR(S2448), and pS6(S235/236) were detected using western blotting. GAPDH served as a loading control (n = 3)
