Fig. 1
Replication of Mtb, MtbΔdosR, MtbΔdosS, and MtbΔdosT in rhesus macaque bone marrow derived macrophages (RhBMDMs) and animal models. a CFU assay in RhBMDMs showing growth progression of Mtb, MtbΔdosR, MtbΔdosT, and growth reduction of MtbΔdosS. The reductions in CFU numbers of MtbΔdosS are statistically significant (***P = 0.00116399, P = 0.00342701, P = 0.00114028 using t-test at 24, 48, and 72 h. b, c Confocal microscopy. Visual comparison of MtbΔdosS (b) and Mtb (c) (red), and RhBMDMs (blue) at 0 and 24 h post infection. Scale bars 20 μm in panels b and c. d Quantitation of bacilli within macrophages under a fixed magnification using a TCS-SP2 confocal microscope (Leica Microsystems) (obtained from panels b, c). e Macrophage response to Mtb and mutant strains using rhesus macaque specific microarrays. Relative differences in immune responses in significance were plotted as negative logarithms (to the base 10) of P-values for Mtb (red), MtbΔdosR (black), MtbΔdosS (blue), and MtbΔdosT (green). f Various signaling pathways obtained using DAVID. For Statistics, unpaired Student’s t-test (parametric test) was performed using SAS 9.2 (SA Institute, Cary, NC). The data are shown from three biological replicates. Scale bar is 20 μm and applies to all images in panels b and c
