Fig. 5

Spontaneous activities of excitatory neurons are less correlated with locomotion. a Locomotion (red) and stationary (blue) periods. b Example fluorescent traces of four excitatory neurons during locomotion and stationary periods. Gray shading indicates bouts of locomotion. Spontaneous activities of the first two neurons are enhanced by locomotion, whereas the last two neurons are suppressed by locomotion. c Spontaneous activities are plotted for the 32 individual running trails (color map) and averaged trails (black lines). Vertical gray bars show onsets of locomotion. d Across the populations, the spontaneous activities of 71% and 29% of excitatory neurons are significantly enhanced and suppressed by locomotion, respectively (n = 312 neurons from 6 FOVs of 6 mice). L locomotion, S stationary. e Individual excitatory neurons are weakly correlated with locomotion. f Weak pairwise correlations among the excitatory neurons are observed during locomotion