Fig. 5 | Communications Biology

Fig. 5

From: Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake

Fig. 5

sEV-VEGF predominantly comprises dimeric VEGF189. a Immunoblot of cellular VEGF in lysates of cells of parental cancer cell lines that were treated with brefeldin A to block protein secretion. Recombinant VEGF proteins were included as controls. Overexposure shows VEGF121 and VEGF165 dimers. b Immunoblot of VEGF in sEVs isolated from the same cell lines as in a but without brefeldin A treatment. c Immunoblot of VEGF in tumor tissues (T) of three patients with ovarian cancer and in sEVs isolated from ascites of the same patients. d Immunoblot of VEGF in sEVs isolated from serum or plasma of five patients with colorectal carcinoma (CRC) and two patients with renal cell carcinoma (RCC). TSG101 was assayed as a control in c, d

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