Fig. 3
From: Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo
Overview of P. renovo nuclear transformation. a Construct design showing genetic elements and primers used to generate DNA for electroporation (49 and 11) and subsequent PCR confirmation of transformants. b PCR verification of 12 clones utilizing primers shown in panel a. c Dot plot of fluorescent plate reader data of wild type and mCherry transformants, normalized to chlorophyll autofluorescence. Data is from three biological replicates. d Confocal microscopy images of wild type and transformant microalgae expressing mCherry. Green coloring represents chlorophyll autofluorescence, red coloring represents mCherry fluorescence, 10 µm scale bar
