Fig. 4
From: Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo
Overview of P. renovo chloroplast transformation. a Construct design showing genetic elements utilized and homology directed integration into the chloroplast genome, along with the primers used for subsequent PCR confirmation of transformants. b PCR verification of 3 clones utilizing primers shown in panel a. c Dot plot of fluorescent plate reader data of wild type and sfGFP transformants, normalized to chlorophyll autofluorescence. Data is from three biological replicates. d Epifluorescent microscopy images of wild type and sfGFP transformant microalgae. Red coloring represents chlorophyll autofluorescence, green coloring represents sfGFP fluorescence, 10 µm scale bar
