Fig. 1

Phylogenetic relationship of AcPT1 to the UbiA superfamily. A phylogenetic tree of members of the UbiA superfamily was constructed by the maximum likelihood method with 1000 bootstrap tests. Bootstrap values are shown for nodes separating clades. Clades related to primary metabolism are shown in gray, and each clade involved in specialized (secondary) metabolism was highlighted in color depending on the closest primary metabolism clade; i.e., orange, green, and blue for neighbors of tocopherol, plastoquinone, and ubiquinone clades, respectively. Gray circles represent Asteraceae PT polypeptides used as queries during in silico screening of AcPT1. The plant origin and accession number of each PT in the tree are described in Supplementary Tables 2 and 3