Fig. 2 | Communications Biology

Fig. 2

From: Isolation of Artemisia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast

Fig. 2

In planta expression and subcellular localization of AcPT1. a, b Gene expression levels of AcPT1 relative to Ac26S rRNA (a) and the contents of three phenylpropane molecules, p-coumaric acid, drupanin, and artepillin C (µg g-fresh weight−1) (b) in leaves, stems, and flowers of A. capillaris. The expression levels of AcPT1 in aerial organs were normalized relative to 26S rRNA and divided by the normalized expression level in leaves. Values (n = 5 biological replicates, except for stems (n = 4) in a are shown as box plots (center line, median; box limits, first and third quartiles; whiskers, minimum and maximum). In either a or b, clear significant differences between pairs were not observed by Tukey HSD test (p > 0.05). c Subcellular localization of AcPT1TP-sGFP in onion cells. Microscopic examination of onion cells co-expressing sGFP or AcPT1TP-sGFP together with WxTP-DsRed. As for AcPT1TP-sGFP, regions indicated by white arrows are enlarged. For merging, brightness and contrast of fluorescent images are unbiasedly adjusted with magenta used as a pseudo-color for the DsRed signal. Scale bars represent 100 µm

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