Fig. 3 | Communications Biology

Fig. 3

From: Isolation of Artemisia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast

Fig. 3

HPLC analysis of the p-coumaric acid:dimethylallyltransferase activity of recombinant AcPT1. a Components used in assays of p-coumaric acid dimethylallyltransferase activity of AcPT1. b HPLC chromatograms of enzyme reaction products. Microsomes prepared from N. benthamiana leaves expressing AcPT1 were used as crude enzymes. UV chromatograms at 315 nm of extracts from the reaction mixture are shown. The negative control consisted of microsomes prepared from N. benthamiana leaves expressing transit peptide-fused sGFP (AcPT1TP-sGFP). c, d Time (c) and enzyme-to-substrate (d) dependences of drupanin (orange circles) and artepillin C (red circles) synthesis and artepillin C per total yield (drupanin + artepillin C) ratios (gray circles). In both c and d, microsomes were incubated with 50 µM of p-coumaric acid and 0.50 mM of DMAPP. Values indicate means ± standard errors (n = 3 independent experiments)

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