Fig. 4
Interaction zones IZ1, IZ2, and IZ3. a Schematic depiction of interaction zones IZ1 (pink), IZ2 (green) and IZ3 (blue) and the corresponding H-bond, hydrophobic and aromatic interactions with (R)-1 (red). b IC50 shift factors and dose-response curves resulting from percental inhibition ± SEM of ifenprodil (blue), (R)-1 (red), (S)-1 (purple) and 2 (green) at oocytes expressing mutated NMDARs GluN1-1a S132A. Curves were generated from recordings of n = 18 independent oocytes (see Supplementary Tables 2–5). c Dose-response curves resulting from percental inhibition ± SEM of rac-1 (gray), 3 (green) and 4 (pink) at GluN1-1a/GluN2B expressing oocytes. Curves were generated from recordings of n = 24 (rac-1) or n = 18 (3, 4) independent oocytes (see Table 1). d, e IC50 shift factors and dose-response curves resulting from percental inhibition ± SEM of ifenprodil (blue), (R)-1 (red), (S)-1 (purple) and 2 (green) at oocytes expressing mutated NMDARs with GluN1-1a L135N (d) and GluN2B E236A (e). Curves were generated from recordings of n = 15–18 independent oocytes (see Supplementary Tables 2–5). f Inhibition data of 30 µM rac-1 (grey), 3 (green), 4 (pink), and 5 (brown) at oocytes expressing wildtype GluN1-1a/GluN2B NMDA receptors presented as single data points (n = 3 independent oocytes per compound) and mean (black bar). g, h IC50 shift factors and dose-response curves resulting from percental inhibition ± SEM of ifenprodil (blue), (R)-1 (red), (S)-1 (purple), and 2 (green) using oocytes expressing mutated NMDARs with GluN1-1a Y109S (g) or GluN2B F114A (h). Curves were generated from recordings of n = 12–18 independent oocytes for each compound (see Supplementary Table 2–5). i Ki values of rac-1, 6, and 7 presented as single data points (n = 3 independent experiments) and mean (black bar). j Dose-response curves resulting from percental inhibition ± SEM of rac-1 (grey), 6 (turquoise), and 7 (blue) at wildtype GluN1-1a/GluN2B NMDARs. Curves were generated from recordings of n = 24 (rac-1) or n = 12 (6, 7) independent oocytes (see Table 1)
