Fig. 5
Interaction zones IZ4 and IZ5. a Schematic depiction of interaction zones IZ4 and IZ5 displaying hydrophobic, aromatic and H-bond interactions with (R)-1 (red). b–d IC50 shift factors and dose-response curves resulting from percental inhibition ± SEM for ifenprodil (blue), (R)-1 (red), (S)-1 (purple) and 2 (green) using oocytes expressing mutated GluN1-1a/GluN2B receptors with GluN2B Q110A (b), GluN1-1a F113S (c) and GluN2B I111A (d). Curves were generated from recordings of n = 15–18 independent oocytes for each compound (see Supplementary Tables 2–5). e Docking conformation of (R)-1 (CPK coloring with green for C-atoms) at ifenprodil binding site of GluN1-1a (purple)/GluN2B (gray) receptor with residue GluN2B F176 in orange. f IC50 shift factors and dose-response curves resulting from percental inhibition ± SEM for ifenprodil (blue), (R)-1 (red), (S)-1 (purple), and 2 (green) using oocytes expressing mutated NMDARs with GluN2B F176A. Curves were generated from recordings of n = 12 independent oocytes for each compound (see Supplementary Tables 2–5)
