Fig. 7
GluN2B wildtype vs. GluN2B F176A vs. GluN2B F176Y. Ifenprodil binding site of (a–c). GluN1-1a (purple)/GluN2B (gray) receptor with bound (R)-1 and wildtype GluN2B F176 (a, orange), mutated GluN2B F176Y (b, orange) and mutated GluN2B F176A (c, orange). d–f Representative current traces of 1 µM (R)-1 (red bar) inhibiting wildtype (d), GluN2B F176Y (e) and GluN2B F176A NMDARs in presence of 10 µM (S)-glutamate and 10 µM glycine (black bar). g–j Dose-response curves resulting from percental inhibition ± SEM of ifenprodil (g, blue), (R)-1 (h, red), (S)-1 (i, purple) and 2 (j, green) inhibiting GluN2B wildtype, GluN2B F176Y (dark color) and GluN2B F176A (light color) NMDARs. Curves were generated from recordings of n = 12–24 independent oocytes for each compound (see Supplementary Table 2–5)
