Fig. 1
From: Properties and efficient scrap-and-build repairing of mechanically sheared 3’ DNA ends
Analysis of MEDEs using a capillary sequencer. FAM-labeled DNA samples were analyzed using a capillary sequencer after mixing with LIZ500 size standard-supplemented HiDi-formamide (HiDi-LIZ500). Two peaks of 100 and 150 nucleotides from the LIZ size standard, used to calibrate the electropherogram, are indicated by filled triangles. a H4 fraction DNA mixture before reaction. b Merged view of ten results obtained by analyzing H4 fraction DNA mixture independently. c–g Data obtained after different enzymatic treatments indicated on the left. Samples were purified using a DNA clean-up column before mixing with HiDi-LIZ500. T4DP, T4 DNA polymerase; SAP shrimp alkaline phosphatase; E3T4, combined treatments with exonuclease III and T4DP; SAP-T4DP, SAP-treated sample purified and treated with T4DP; E3T4-T4DP, E3T4-treated sample purified and treated with T4DP. In panel g, each peak is labeled with a corresponding base. All data were y-axis scaled so that sums of FAM peak areas are apparently even across the panels
