Fig. 3
From: Properties and efficient scrap-and-build repairing of mechanically sheared 3’ DNA ends
G-tailing and CIS treatments. a Schematic drawing of G-tailing and CIS reactions. G-tailing reaction using MMLV-RT conducted in the presence of 4 mM MnCl2 and 200 mM deoxycytidine appended approximately four deoxyguanosine monophosphates to the 3’-OH terminus. For G-tailing, a single-stranded DNA with serial deoxycytidine monophosphates was incorporated with concomitant DNA polymerization mediated by MMLV-RT. Asterisk (*) indicates 5’ FAM fluorophore. b H4 fraction DNA before reaction (identical to that shown in Fig. 1a). c–e 50 fmol of H4 DNA fraction was subjected to serial reactions as indicated. Between the two enzymatic treatments, and before mixing with HiDi-LIZ500, the samples were purified using the DNA clean-up column
