Fig. 5
Real-time fluorescence imaging of newly acidified organelles during autophagy. a Hela cells were loaded with 1 (10 μM) for 30 min and were submitted to confocal microscope observation followed by addition of rapamycin (10 μM) under microscope. For each field, a series of 10–20 serial focal plane images was recorded, serial focal planes were 0.8 μm apart and were illuminated. Fluorescence images after selected time intervals, scale bar 10 μm. b Histogram of fluorescence intensity vs time upon addition of rapamycin. c Histogram of fluorescence intensity vs time without addition of rapamycin, scale bar 10 μm.
