Fig. 4: IRF1 and IRF3/7 control unique gene expression signatures upon STING activation.
From: T cell fate following Salmonella infection is determined by a STING-IRF1 signaling axis in mice
RNA-seq analyses of BMDCs from wild-type, Tmem173−/−, Irf1−/−, and Irf3/7−/− mice 18 h after 50 μg/ml of c-di-GMP stimulation. a Venn diagram of at least two-fold differentially expressed genes (DEGs) in the indicated pairwise cuffdiff analysis from wild-type, Tmem173−/−, Irf1−/−, or Irf3/7−/− BMDCs. DEGs were identified using an FDR cutoff <0.05 and a fold change cutoff >2. b GSEA/MisDB analysis showing significant pathways in “Hallmark” and “Canonical pathway” categories. c Starwars plot generated in Seqmonk showing the expression of DEG signatures in CDG-dependent gene clusters in wild-type, Tmem173−/−, Irf1−/−, or Irf3/7−/− BMDCs. The individual data points are given in the supplemental data sets for the identified clusters (Supplementary Data 1). For each group the mean quantification of gene signatures is represented by a filled circle and the confidence interval (standard error) presented by error bars. The Heat map shows the hierarchical clustering of DEGs from wild-type, Tmem173−/−, Irf1−/−, and Irf3/7−/− BMDCs stimulated for 18 h by c-di-GMP. d–f Heat maps showing genes contained in the indicated clusters in c that contain IRF1 and IRF3/7-dependent gene signatures with key regulators high-lighted in red.
