Fig. 1: In vitro reaction experimental overview.
From: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
A Cas12a or Cas9 RNP is combined with plasmid DNA, a cell-free extract and a donor DNA template in an in vitro reaction mixture. The DNA is then recovered from the in vitro reaction and transformed into competent E. coli. cells. Bacterial colonies are selected individually for sequencing and mutational analysis. The entirety of this process takes ~16 h to complete.
