Fig. 2: In vitro targeting and potential outcomes.
From: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
a The target sites for Cas9 and Cas12a are shown along the lacZ gene with corresponding 1364-S and 1364-NS HDR templates. b An illustration is shown of the potential outcomes of in vitro reaction products. c An agarose gel is shown to display the cleavage of pHSG299 plasmid DNA by Cas9 and Cas12a at increasing pmol amounts in the in vitro system.
