Table 1 Frequencies of various reaction outcomes.

From: Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair

 

Total

HDR

Indel

Cas9 1364-S

37

2 (5.4%)

35 (94.6%)

Cas9 1364-NS

34

6 (17.6%)

28 (82.4%)

Cas12a 1364-S

32

6 (18.8%)

26 (81.2%)

Cas12a 1364-NS

35

23 (65.7%)

12 (34.3%)

  1. The total number of bacterial colonies selected from each of the four unique reaction conditions for individual sequencing and mutational analysis is shown. The number and frequencies of HDR and Indel events seen within each reaction condition are displayed.
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