Fig. 1: Molecular phylogeny and structure of aquaporin genes.
From: The vertebrate Aqp14 water channel is a neuropeptide-regulated polytransporter
a Mid-point rooted maximum likelihood tree (heuristic search optimised for parsimony; GTR model: NST = 6; partitioned by codon) of the major aquaporin subfamilies in Sarcopterygii represented by the platypus (magenta branches) and Actinopterygii represented by zebrafish (blue branches) illustrating the position of the AQP14 subfamily next to AQP4 in the classical grade of aquaporins. Since the AQP10 gene is not currently found in the platypus genome, the metatherian Tasmanian devil AQP10 ortholog is included. Bayesian inference (1,000,000 MCMC generations) yielded the same tree toplogy with posterior probability ranges indicated at each node. b Summarised Bayesian majority rule consensus tree (25 million MCMC generations inferred from 170,646 nucleotide sites) of aqp14 channels from 59 orders of fishes. The tree is rooted with lamprey aqp14. R4 represents the fourth round of whole-genome duplication in salmonids and some cyprinids, with the salmonid aqp14_1 pseudogenes† indicated in red. Key denotes the level of posterior probability at each node. Scale bar represents the expected substitutions per site. c Gene structures drawn to scale for representative species in each order. d Syntenic blocks for representative species in each order. Pointed ends of the gene symbol indicates coding direction, solid line indicates full linkage, dashed line indicates absence of scaffold data. Red gene symbols with a cross indicate pseudogenes. Arrows highlight lineages selected for functional experiments.
