Fig. 3: Expression of Aqp14 in different teleost tissues.
From: The vertebrate Aqp14 water channel is a neuropeptide-regulated polytransporter
a–c Representative RT-PCR analysis of aqp14 gene expression in different tissues from zebrafish (a), Atlantic salmon (b) and gilthead seabream (c) (N = 3 fish as indicated). The minus indicates absence of RT during cDNA synthesis. The size (kb) of PCR products and molecular markers are indicated on the left. Uncropped gels are shown in Supplementary Fig. 2. d–f Representative immunoblots of Aqp14, and prohibitin (Phb) as loading control, in protein extracts from brain, eye, lens, testis, rectum and gills from zebrafish (d), Atlantic salmon (e) and gilthead seabream (f) using the SaAqp14 antibody. In each blot, arrows indicate aquaporin monomers based on in silico determination of molecular masses of DrAqp14 (29.5 kDa), SsAqp14 (29.4 kDa) and SaAqp14 (29.3 kDa). Duplicated blots were run in parallel and incubated with the primary antibody preadsorbed by the antigenic peptide to test for specificity. Molecular mass markers (kDa) are on the left. Uncropped blots are shown in Supplementary Fig. 3d, e.
