Fig. 4: Immunolocalization of Aqp14 in different tissues from zebrafish, Atlantic salmon and gilthead seabream.
From: The vertebrate Aqp14 water channel is a neuropeptide-regulated polytransporter
a–g Representative brightfield and immunofluorescence microscopy images of Aqp14 in the seabream and zebrafish optical tectum of the brain (a and b, respectively), zebrafish lens (c) and retina (d), seabream gills (e) and testis (f), and Atlantic salmon muscle (g). Sections were labeled with affinity-purified seabream Aqp14 antiserum (red) and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue) and wheat germ agglutinin (WGA) (green). h–n Control sections incubated with preabsorbed antiserum were negative. Scale bars: 20 µm (10 µm insets). SM stratum marginale, SO stratum opticum, SFS stratum fibrosum superficiale, SPV stratum periventriculare, SGC stratum griseum centrale, C capsule, LE lens epithelium, LF lens fiber, ONF optic nerve fiber, GC ganglion cells, INL, inner nuclear layer, ONL outer nuclear layer, GA gill arch, EC epithelial cell, GL gill lamellae, TA tunica albugínia, SPG spermatogonia, SPZ spermatozoa, WMF white muscle fiber, MY myocyte.
