Fig. 2: In anisotropic substrates, twines emerge from actin ruffles and engage with neighboring fibers.
From: Force-exerting perpendicular lateral protrusions in fibroblastic cell contraction
Time-lapse images showing (a) actin wave (green arrow) traveling along the length of the cell body (Movie M7). b Ruffles from the wave develop into striated structures that evolve into nano-filamentous nascent ‘twines’ shown by red arrowheads (Movie M8). c (i) Image of a cell with a primary twine pointed by the yellow arrow. (ii–vi) Time-lapse images of area indicated by dashed oval demonstrating that twine engagement with neighboring fiber takes place in ~2 s of intial primary twine contacting a neighboring fiber ((iv–vi), Movie M9). The green arrow shows growth of primary twine from cell body and blue arrow shows twine growth after engagement with neighboring fiber i.e. overhanging part of twine. Scale bar: a 20 μm, b 5 μm, c 5 μm.
