Fig. 4: Actin kinetics in PLP formation.
From: Force-exerting perpendicular lateral protrusions in fibroblastic cell contraction
a Twines attached to neighboring fibers grow in width along the neighboring fiber axis as demonstrated by the kymograph. Kymograph was obtained by performing a line scan along the fiber axis. The average displacement vs. time profiles show twine advancement before and after engagement with neighboring fibers (n = 17 for both). The inset shows average velocity for the two categories. b Displacement vs. time profiles showing extension rates of actin lamellum between twine-bridges (n = 13). Inset shows cartoon of actin lamellum advancement along the twine-bridge. c Broadening of twine-bridge into force exerting PLP (n = 15). Linear fits to average of all profiles is included in each plot. In a–c representative immunostained f-actin images are included to show distinct geometries of twines and 3D-PLPs. Scale bar 20 microns.
