Fig. 7: Overexpression of CRPC-Lnc #6 promotes cell growth and AR expression by locating splicing factor U2AF2 in the nucleus. | Communications Biology

Fig. 7: Overexpression of CRPC-Lnc #6 promotes cell growth and AR expression by locating splicing factor U2AF2 in the nucleus.

From: Identification of long non-coding RNAs in advanced prostate cancer associated with androgen receptor splicing factors

Fig. 7

a Overexpression of CRPC-Lnc #6 (Lnc #6) in LNCaP cells. LNCaP cells stably expressing CRPC-Lnc #6 were established. Expression level of CRPC-Lnc #6 was determined by qRT-PCR analysis (N = 3). Values represent mean ± S.D. b Overexpression of CRPC-Lnc #6 (Lnc #6) promotes cell growth. Cell growth assay by counting viable cells were performed (N = 4). Values represent mean ± S.D. Two-way ANOVA was performed to determine the P value. c Western blot analysis of AR expression in LNCaP cells overexpressing CRPC-Lnc #6 (Lnc #6). d Expression level of AR mRNA was determined by qRT-PCR analysis (N = 3). IB immunoblot. Values represent mean ± S.D. Two-way ANOVA was performed to determine the P value. e Combinational RNA-FISH and immunofluorescence analysis to detect U2AF2 distribution and CRPC-Lnc #6 expression. Results of LNCaP cells overexpressing CRPC-Lnc #6 (Lnc #6-2) or control cells (Vec-1) are shown. Bar = 10 μm. f Western blot analysis of nuclear U2AF2 expression in LNCaP cells overexpressing CRPC-Lnc #6. H3 was used as a loading control of nuclear fraction of proteins. IB immunoblot. g Working model of AR and AR-regulated CRPC-Lncs in prostate cancer progression.

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