Table 1 Standard membrane feeding assay results for Pfs230D1-specific mAbs.

From: Structure and function of a malaria transmission blocking vaccine targeting Pfs230 and Pfs230-Pfs48/45 proteins

mAb

Isotype

Exp No.

TB activity with human complement

Inf/Dissa

Avg No. oocysts

Median No. oocysts (range)

% reduction oocysts

% reduction prevalence

3G2

IgG2b

1

20/20

59.6

62 (21–110)

16.4

0

 

6

21/21

62.8

58 (14–139)

−0.7

0

5G3

IgG1

1

20/20

68.6

66 (20–115)

3.7

0

 

6

21/21

47.9

39 (8–99)

23.2

0

5H1

IgG1

1

2/20

0.1

0 (0–1)

99.9

90

 

2

0/20

0

0 (0–0)

100

100

 

3

15/20

1.8

1 (0–6)

93.2

25

 

6

21/22

4.5

4 (0–13)

92.9

4.6

7G4

IgG1

6

21/21

74.2

68 (6–214)

−19

0

 

7

20/20

46

47.5 (1–83)

9.3

0

4F12b

IgG1

1

20/20

39.4

38 (8–76)

42.2

0

 

2

15/20

8.4

0 (0–38)

67.1

25

4F12(3B6)

 

4

21/21

59.6

55 (4–118)

40.3

0

 

6

22/22

33.8

30 (1–64)

45.9

0

rh4F12b

mFab/

5

17/20

5.4

3.5 (0–27)

92.2

15

hIgG1 Fcc

8

19/20

8.1

5.5 (0–19)

84.3

5

  1. m murine, h human, TB transmission-blocking.
  2. aInfected/Dissected.
  3. bPercent reduction in oocysts by rh4F12 compared to 4F12 is significantly different (p = 0.0059, by Welch Two Sample t-test); mAbs were tested at ~1.0 mg/mL IgG except Exp. 8 which was assessed at 0.6 mg/mL.
  4. cPositive and negative controls for this assay are shown in Supplementary Table 2.
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