Fig. 2: Effect comparison between cDNA-normalized and non-normalized SMS in diversity of captured isoforms. | Communications Biology

Fig. 2: Effect comparison between cDNA-normalized and non-normalized SMS in diversity of captured isoforms.

From: Improving the diversity of captured full-length isoforms using a normalized single-molecule RNA-sequencing method

Fig. 2

a Overall difference in the diversity of captured full-length high-quality isoforms (normalized as per 100k total ROIs). Fold change of the isoform number was indicated in purple. b Difference in the diversity of captured isoforms with varied gene expression levels. c Occupancy percentages of ROIs for the captured isoforms with varied gene expression. The percentages were indicated on the top of each bar. In b, c The isoform represented genes were binned according to their expression as measured by FPKM of SGS data. The SGS, normalized, and non-normalized SMS data of human peripheral blood samples were used for the analysis.

Back to article page