Fig. 3: Effect comparison between cDNA-normalized and non-normalized SMS in completeness of captured isoforms. | Communications Biology

Fig. 3: Effect comparison between cDNA-normalized and non-normalized SMS in completeness of captured isoforms.

From: Improving the diversity of captured full-length isoforms using a normalized single-molecule RNA-sequencing method

Fig. 3

a Length distribution of ROIs, high-quality isoforms and full-length high-quality isoforms. The distribution of distance to annotated TSSs (aTSS, left) and annotated TTSs (aTTS, right) was shown in b, d for ROIs and full-length high-quality isoforms, respectively. In a, b, d the normalized and non-normalized libraries were shown in magenta and black, respectively. c The composition of high-quality isoforms. The high-quality isoforms retained as full-length high-quality ones were shown in green, whereas the filtered-away ones because of redundancy or 5′-degradation were shown in gray. Data of human peripheral blood samples were used for the analysis.

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