Fig. 10: Western blot indicating the presence of PK-resistant material in PMCA reaction (+) and non-sonicated control (−) samples of PrPSc amplified with Tg35 (huPrP G127/M129), Tg152 (huPrP G127/V129) and Tg183 (huPrP V127/M129) brain homogenate as substrate. | Communications Biology

Fig. 10: Western blot indicating the presence of PK-resistant material in PMCA reaction (+) and non-sonicated control (−) samples of PrPSc amplified with Tg35 (huPrP G127/M129), Tg152 (huPrP G127/V129) and Tg183 (huPrP V127/M129) brain homogenate as substrate.

From: Structural effects of the highly protective V127 polymorphism on human prion protein

Fig. 10

In each case, a small amount of seed can be detected in the non-sonicated control samples with varying levels of amplification observed in the reactions with different substrates. Of note is the lack of amplification with V127/M129 PrP (Tg183) as substrate.

Back to article page