Fig. 3: Reproducible measurement of SEphA scores.
From: Single-cell analysis of EphA clustering phenotypes to probe cancer cell heterogeneity
a Distributions of SEphA scores for PEO1 and HeyA8 lines. Every PEO1 cell displays very scattered puncta. It is reflected by the low average SEphA score for the population and a distribution with a small spread. HeyA8 population presents a large intercellular variability in clustering morphologies that is reflected in the high population score and a distribution with a large spread. The matching between clustering morphologies and SEpHA scores is exemplified for PEO1 (seven cells) and HeyA8 (eight cells) lines (Scale bar: 5 µm). b The inter-chip (six chips) variability of the score distribution for MDA MB231 (top) and HN137m (bottom) cells shows significant differences between replicates. c Normalization of all distributions on individual chips by the average scores of triplicates of the reference cell line MDA MB231 abolishes significant inter-chip variability. It allows quantitative comparison between chips and cell types. Each distribution is based on N > 200 single-cell analysis. We established significance using the two-sample Kolmogorov–Smirnov test that probes for changes in the shape of the distribution.
