Fig. 2: zDHHC5 interacts with intracellular loop 3 of the Na-pump α-sub-unit. | Communications Biology

Fig. 2: zDHHC5 interacts with intracellular loop 3 of the Na-pump α-sub-unit.

From: Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase

Fig. 2

a A series of peptides covering the PLM C-tail successfully purified endogenous Na-pump α sub-unit but not zDHHC5 from rat ventricular myocyte lysate. b Whole rat heart homogenate was lysed with four different detergents (C12E10, Triton X-100, SDS, and FC-12). Each lysate was split in half and two separate purifications performed using the NBSP or an antibody specific to PLM phosphorylated at serine 68. c Co-expression of HA-zDHHC5 in FT-293 cells stably expressing human PLM caused an increase in FLAG-IL3 accumulation compared to when FLAG-IL3 was expressed alone (n = 5: **p < 0.01). d HA-tagged zDHHC5 can be purified from a HEK cell lysate using recombinant GST-IL3 but not GST alone or GST-IL4.

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