Fig. 4: zDHHC5 GlcNAcylation regulates PLM palmitoylation. | Communications Biology

Fig. 4: zDHHC5 GlcNAcylation regulates PLM palmitoylation.

From: Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase

Fig. 4

a Affinity purification of zDHHC5 with recombinant HALO-tagged catalytically inactive OGA from both HEK cells (upper) and adult rat ventricular myocytes (lower). UF unfractionated lysate, P OGA purified fraction. b A FT-293 stable cell line expressing human PLM was transfected with HA-tagged zDHHC5 or a GlcNAcylation-deficient mutant (S241A) and treated overnight +/− 1 µM GNSG (an OGA inhibitor). Relative PLM palmitoylation (Palm/UF) was determined by acyl-RAC (n = 6: *p < 0.05, ***p < 0.001). c From the same unfractionated samples, zDHHC5 was also immunoprecipitated using HA beads, and the amount of associated PLM determined by Western Blotting (n = 4: *p < 0.05, **p < 0.01).

Back to article page