Fig. 5: Selective pharmacological manipulation of PLM palmitoylation. | Communications Biology

Fig. 5: Selective pharmacological manipulation of PLM palmitoylation.

From: Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase

Fig. 5

a HEK cells stably expressing human PLM were incubated with 30 µM of a stearate-tagged peptide (either NBSP (DP) or scrambled NBSP (S)) for a period of 3 or 24 h. Following cell lysis, relative PLM and Ras palmitoylation (Palm/UF) values were determined using a combination of Acyl-RAC and Western Blotting (PLM: n = 6; Ras: n = 4; **p < 0.01, ****p < 0.0001). b The same treatments were also applied to adult rat ventricular myocytes prior to determining the palmitoylation status of both PLM and Cav3 (PLM: n = 6; Cav3: n = 4; *p < 0.05).

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