Fig. 4: Weighted gene co-expression network analysis (WGCNA) across developmental stages for the screwworm, C. hominivorax.
a Average linkage hierarchical clustering dendrogram of the genes. The expression distance is shown in the y-axis. Modules, designated by color code in the x-axis, are branches of the clustering tree. These modules are made of genes with a high correlation of their expression levels across the developmental stages. b Relationship between each module based on similarity of expression between all genes for each developmental stage. The light yellow color show high topology overlap (high interconnection), and the dark color represents low overlap (high interconnection). c Correlation of gene expression patterns for each module eigengenes in relation to developmental stages. Each row corresponds to a module eigengene and columns are developmental stage. Asterisk represents values with a significant positive correlation for based on a regression-based p-value for assessing the statistical significance (P < 0.05) between a stage and module91. Some modules are correlated with specific developmental stages. Gene ontology (GO) analysis of eigengenes associated with each stage was conducted with g:Prolifer89. Frequencies represent the relative number associated with the specific GO compared to the GOA database93.
