Fig. 7: gdPT induces an activated memory response in an autologous human whole-blood (hWB) culture. | Communications Biology

Fig. 7: gdPT induces an activated memory response in an autologous human whole-blood (hWB) culture.

From: Genetically detoxified pertussis toxin displays near identical structure to its wild-type and exhibits robust immunogenicity

Fig. 7

Mass cytometry was used to analyze whole-blood cells cultured for 7 days following stimulation with either PTd, gdPT, or a mock control (media alone). A tSNE-based dimension reduction analysis with viSNE was performed on CD45+CD66b− cells, showing the difference in cell population distribution following whole-blood stimulation. Every dot in the viSNE plot represents a single cell with an associated phenotype. a Cell populations defined by manual gating (Supplementary Fig. 6) projected onto the viSNE plots; each cell population is assigned a specific color. The CD123+CD11c− HLA-DR− pDC were identified and gated in viSNE plot based on the signal intensity of the cell surface markers CD123, CD11c, and HLA-DR. b A sun-burst plot representing the relative abundance (percentage of CD45+CD3+ events) of T cell populations following whole-blood stimulation. The concentric rings represent the gating hierarchies used and the sizes of the wedges are proportional to the abundance of the populations.

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