Fig. 6: PRMT5 regulates cell survival after DNA damage. | Communications Biology

Fig. 6: PRMT5 regulates cell survival after DNA damage.

From: PRMT5 promotes DNA repair through methylation of 53BP1 and is regulated by Src-mediated phosphorylation

Fig. 6

a–d MCF7 cells were transfected with siRNA (a, b) or expression vector (c, d) as described, and then exposed to etoposide for 2 h. After etoposide exposure, cells were incubated with fresh media for 2 weeks. Cell colonies were stained with Crystal violet. a, c Representative picture of colonies from three independent experiments. b, d Analysis of colony-forming rate by measurement of absorbance at 540 nm. Data are means ± SD of three independent experiments (*P < 0.05 and **P < 0.01). e A model for the regulation of PRMT5 activity. PRMT5 activity may act as a major determinant of whether the cells undergo DNA repair or apoptosis in response to DNA damage.

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