Fig. 4: Active site is fine-tuned by the acetyl group.

Structural comparison of Dat/CoA complex (yellow, PDB code: 5GI5) and Dat/Ac-CoA complex (blue, PDB code:3TE4) reveals different positions of α7 and loop–α9 changes. Residues are shown as sticks. Salt bridges in Asp46–Arg153 and Lys192–CoA moiety are shown in orange dashed lines.