Fig. 4: Anti-parasitic activity of LGALS-11 isoforms on H. contortus.
From: The oligomeric assembly of galectin-11 is critical for anti-parasitic activity in sheep (Ovis aries)
a The effect of LGALS-11 isoform 1 on H. contortus larval development. Exsheathed larval stages (xL3s) were incubated with LGALS-11 isoform 1 or 2, or carbohydrate recognition domain mutant (CRD-m) or dimer interface mutant (DI-m) for 7 days at 0.009–2.5 mg/ml concentration. L4 development was recorded microscopically. Control larvae were incubated for the same time only in the buffer used to resuspend individual LGALS-11 proteins. b Pathological changes observed in the L4 stage of H. contortus incubated with LGALS-11 isoform 1. Images showing representative phenotypes of L4 larvae following incubation with LGALS-11 isoform 1 or 2, or mutant CRD-m or DI-m for 3 days (1 mg/ml); control larvae were incubated for the same time only in the buffer used to resuspend individual LGALS-11 proteins. Extensive structural damage was observed in larvae incubated with isoform 1 at 1 mg/ml concentration. Scale bar = 20 μm.
